Differentiation of human ESCs to retinal ganglion cells using a CRISPR engineered reporter cell line скачать в хорошем качестве

Differentiation of human ESCs to retinal ganglion cells using a CRISPR engineered reporter cell line

Differentiation of human ESCs to retinal ganglion cells using a CRISPR engineered reporter cell line. Valentin M. Sluch et al (2015), Scientific Reports http://dx.doi.org/10.1038/srep16595 Retinal ganglion cell (RGC) injury and cell death from glaucoma and other forms of optic nerve disease is a major cause of irreversible vision loss and blindness. Human pluripotent stem cell (hPSC)-derived RGCs could provide a source of cells for the development of novel therapeutic molecules as well as for potential cell-based therapies. In addition, such cells could provide insights into human RGC development, gene regulation, and neuronal biology. Here, we report a simple, adherent cell culture protocol for differentiation of hPSCs to RGCs using a CRISPR-engineered RGC fluorescent reporter stem cell line. Fluorescence-activated cell sorting of the differentiated cultures yields a highly purified population of cells that express a range of RGC-enriched markers and exhibit morphological and physiological properties typical of RGCs. Additionally, we demonstrate that aligned nanofiber matrices can be used to guide the axonal outgrowth of hPSC-derived RGCs for in vitro optic nerve-like modeling. Lastly, using this protocol we identified forskolin as a potent promoter of RGC differentiation. Good channel:    / dlium   Subscribe, like and comment. Good website: https://www.dlium.com Bookmark, subscribe and comment.