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Welcome to the best hobby ever: tissue culture Start doing TC today with the Plants in Jars Starter Kit 🌱🧪👩🔬 https://bit.ly/4qdw4QB Shop my Lab on Amazon: https://www.amazon.com/shop/plantsinj... / discord / plantsinjars_tc ____________________________________________ SUPPLIES LIST Magnetic stirrer https://amzn.to/459tOS6 1-liter glass beaker https://amzn.to/4s39vQn Digital scale (0.01 g precision recommended) https://amzn.to/4s1fANg Pour/weigh boats https://amzn.to/499D37f Micropipette (200 - 1000 uL is the size you need) https://amzn.to/4oOEWLf Pipette tips (compatible with your pipette) https://amzn.to/3My9js4 pH probe ("For General Water" model) https://amzn.to/3MGvvA8 pH up & down solutions https://amzn.to/4afnYSF Pressure cooker https://amzn.to/3KT9ZHP Pressure cooker racks https://amzn.to/4pFyvLV Instant Pot (if you don't want to use a pressure cooker) https://amzn.to/4pciE6B Plants in Jars Starter Kit https://bit.ly/4qdw4QB Polypropylene (PP) Containers https://amzn.to/3XVAu2p PP Lids https://amzn.to/4q7mw9w Forceps https://amzn.to/4rVSFmj Scissors https://amzn.to/4rWjJSq Tween 20 https://amzn.to/48TThQQ Culture Racks https://amzn.to/4oOSJ4F LED lights https://amzn.to/49fEEaL Outlet timer https://amzn.to/4q7PK8m ____________________________________________ 00:00 Meet our willing victim (African Violet) 00:55 Kitchen Counter Science 01:36 Protocols 02:43 Making the Forbidden Jello 04:41 Cosplaying breaking bad in my kitchen 05:46 Plant Hormones 06:40 Explaining how to use a pipette 07:28 dealing with pH 08:16 A recent change to my method (updated lore) 08:38 The Benefits of PP and why you should use it 09:14 Pouring the Media 09:52 Pressure Cooking the Media 10:36 Rapid fire Q&A 11:00 Unloading the pressure cooker 11:14 other supplies (you'll see what we need these for later) 11:52 Introducing the Still Air Box 12:46 Collecting the tissue sample 13:28 Washing the leaves 14:08 ADDRESSING MY HATERS 14:33 Bleaching the plants (perfectly ethical btw) 15:17 Rinsing the Explants 15:50 Sterile Transfers 18:00 Tissue Culture HACK 19:04 Lighting & Temperature 19:29 Troubleshooting Common Issues 20:46 Subculturing 21:38 bye ____________________________________________ Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you. ____________________________________________ Track: ILY Baby - Dyalla | No Copyright Musi... Music provided by GameNCM. Channel: / @gamencm Free Download / Stream: https://bit.ly/3BknoRx