Pericytes incorporation into Endothelial Cell Bead Sprouting Assay | Protocol Preview скачать в хорошем качестве

Pericytes incorporation into Endothelial Cell Bead Sprouting Assay | Protocol Preview

Watch the Full Video at https://www.jove.com/v/57309/incorpor.... Incorporating Pericytes into an Endothelial Cell Bead Sprouting Assay - a 2 minute Preview of the Experimental Protocol Salma H. Azam, Mitchell Smith, Vivek Somasundaram, Chad V. Pecot University of North Carolina at Chapel Hill, Curriculum in Genetics and Molecular Biology; University of North Carolina at Chapel Hill, Lineberger Comprehensive Cancer Center; University of North Carolina at Chapel Hill, Department of Medicine; This protocol presents a novel in vitro bead assay that more appropriately models the process of in vivo sprouting angiogenesis by incorporating pericytes. This modification enables the bead assay to more faithfully recapitulate the heterotypic cellular interactions between endothelial cells and mural cells that are critical for angiogenesis. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments