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Watch the Full Video at https://www.jove.com/v/59090/an-ex-vi.... An Ex Vivo Tissue Culture Model for Fibrovascular Complications in Proliferative Diabetic Retinopathy - a 2 minute Preview of the Experimental Protocol Erika Gucciardo, Sirpa Loukovaara, Ani Korhonen, Kaisa Lehti University of Helsinki, Research Programs Unit, Genome-Scale Biology, Biomedicum Helsinki; University of Helsinki and Helsinki University Hospital, Unit of Vitreoretinal Surgery, Ophthalmology; Karolinska Institutet, Department of Microbiology, Tumor, and Cell Biology (MTC); Here, we present a protocol to study the pathophysiology of proliferative diabetic retinopathy by using patient-derived, surgically-excised, fibrovascular tissues for three-dimensional native tissue characterization and ex vivo culture. This ex vivo culture model is also amenable for testing or developing new treatments. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments