Confocal Ophthalmoscopic Imaging and Cell Morphometry in Glaucoma | Protocol Preview скачать в хорошем качестве

Confocal Ophthalmoscopic Imaging and Cell Morphometry in Glaucoma | Protocol Preview

Watch the Full Video at https://www.jove.com/v/52731/in-vivo-.... In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell Morphometry in Mouse Glaucoma - a 2 minute Preview of the Experimental Protocol Alejandra Bosco, Cesar O. Romero, Balamurali K. Ambati, Monica L. Vetter University of Utah, Department of Neurobiology & Anatomy; University of Utah, Department of Ophthalmology & Visual Sciences; Microglia activation and microgliosis are key responses to chronic neurodegeneration. Here, we present methods for in vivo, long-term visualization of retinal CX3CR1-GFP+ microglial cells by confocal ophthalmoscopy, and for threshold and morphometric analyses to identify and quantify their activation. We monitor microglial changes during early stages of age-related glaucoma. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments