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Join Senior Scientists, Madeline Smith and Lloyd Jones from Ecolab Life Sciences as they demonstrate the proper technique for packing a Bioprocess Glass (BPG) chromatography column. In this instructional video, our expert presenters guide you through each crucial step of the process, ensuring optimal column performance for your purification needs. Presenters Maddie Smith, Senior Chemist Lloyd Jones, Chemist Key Topics Covered Equipment preparation and column assembly Resin preparation and slurry transfer Packing process and flow rate control Column stabilization and equilibration Quality control tests (HETP and asymmetry) Sanitization, storage, and maintenance tips Benefit from the expertise of Maddie and Lloyd as they share insights and best practices throughout the demonstration. This video is essential for laboratory technicians, bioprocess engineers, and researchers working with chromatography systems. By following these steps, you'll achieve consistent and efficient column packing, crucial for successful purification processes. Website - https://www.purolite.com/index LinkedIn - @ / purolite Introduction and Content, 0:00 Terminology, 0:51 Equipment Required, 1:40 Slurry Concentration Calculation, 1:56 Determination of Slurry Volume, 2:25 Determination of Slurry Volume for BPG, 2:53 Column Preparation, 3:12 Addition of Resin to the Column, 3:50 Mixing the Resin, 4:19 Installation of the Top Adaptor, 5:01 Securing the Top Adaptor, 5:42 Lowering the Adaptor, 6:13 Attaching Tubing, 6:48 Packing Pressure Setting, 7:22 Marking the Bed Height, 8:26 Lowering the Adaptor, 8:58 Column Efficiency Testing and Calculations, 9:48 Further Resources, 12:05