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Watch the Full Video at https://www.jove.com/v/57474/protocol.... Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting - a 2 minute Preview of the Experimental Protocol Frauke Hausburg, Paula Müller, Natalia Voronina, Gustav Steinhoff, Robert David Rostock University Medical Center, Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery; Rostock University, Department Life, Light and Matter of the Interdisciplinary Faculty; This protocol illustrates a safe and efficient procedure to modify CD133+ hematopoietic stem cells. The presented non-viral, magnetic polyplex-based approach may provide a basis for the optimization of therapeutic stem cell effects as well as for monitoring the administered cell product via magnetic resonance imaging. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments