Introduce Your Students to Gene Editing with CRISPR (featuring the Edvotek EDGE) скачать в хорошем качестве

Introduce Your Students to Gene Editing with CRISPR (featuring the Edvotek EDGE)

This is a replay of our biotechnology training workshop which was held on May 20 at 3:30 PM EDT. Modern biotechnology encompasses a variety of techniques that are used extensively in today’s labs, such as genetic engineering and creating recombinant DNA techniques. These advances allow scientists to directly manipulate DNA sequences, dramatically reducing the time necessary to study and improve organisms. In the past 10 years, researchers have begun to use the CRISPR-Cas9 system to genetically modify DNA sequences. The development of this system as a gene editing tool is one of the most exciting biotechnology breakthroughs of the past decade. In this workshop, we will explore CRISPR biology using the Edvotek EDGE in a fast, easy experiment that models the development of a cure for Cystic Fibrosis. Experiments featured: Using CRISPR To Treat Cystic Fibrosis: https://www.edvotek.com/135 CRISPR Origami Organelle: https://www.edvotek.com/EVT-031 For all Edvotek CRISPR offerings: https://www.edvotek.com/Experiments/C... Equipment featured: The NEW Edvotek EDGE: https://www.edvotek.com/500 Variable Micropipette 20 - 200 µl: https://www.edvotek.com/591-1 For the slides: https://www.edvotek.com/site/pptx/edv... ______________________________ Don't forget to like and subscribe to our social media! YouTube:    / edvotekinc   Twitter:   / edvotek   Instagram:   / edvotek     / edvotek   _______________________________ Modern biotechnology encompasses a variety of techniques that are used extensively in today’s labs, such as genetic engineering and creating recombinant DNA techniques. These advances allow scientists to directly manipulate DNA sequences, dramatically reducing the time necessary to study and improve organisms. In the past 10 years, researchers have begun to use the CRISPR-Cas9 system to genetically modify DNA sequences quickly, cheaply, and precisely. The development of this system as a gene editing tool is one of the most exciting biotechnology breakthroughs of the past decade. The basics of CRISPR were discovered over the past 30 years or so, starting with the identification and characterization of the system in bacteria. In the early 2010’s, three research groups began to develop CRISPR into a gene editing tool -- Jennifer Doudna at the University of California in Berkeley who worked on microbial CRISPR-Cas systems; Emmanuelle Charpentier, then at the University of Vienna in Austria, who also worked on microbial CRISPR-Cas systems; and Feng Zhang at the Broad Institute of MIT who pioneered CRISPR systems in mammalian and human cells. They recognized that using this system, we could can find any short sequence of DNA and attack it with precision. All three of these scientists contributed to the research that made CRISPR a real research tool and not just an interesting phenomenon. Now, there’s a lot of science drama surrounding CRISPR and the patent situation – the applications of the technology, the development of the component parts, and more. I’m not going to cover a lot of it in this presentation, but it would be an interesting thing to have your students research and explore as an independent study, or to provide some articles for reading and discussion. But, for their contributions, Doudna and Charpentier won the 2020 Nobel Prize in Chemistry! In this workshopw, we talk about CRISPR using both an agarose gel electrophoresis simulation and an Origami Organelle. While we won’t be performing actual CRISPR, which can take several weeks in the classroom, through this fun and easy simulation, students learn how CRISPR-Cas9 can target and “fix” a specific mutation. And we do this through designing guide RNAs that are used by CRISPR-Cas9 to target mutations in the gene that causes cystic fibrosis, a rare genetic disorder.