Part 1– Yeast Two-Hybrid Assay & Setup | DNA-Binding Domain(DBD) & Activation Domain(AD)-Bait & Prey скачать в хорошем качестве

Part 1– Yeast Two-Hybrid Assay & Setup | DNA-Binding Domain(DBD) & Activation Domain(AD)-Bait & Prey

‪@BiologyWithChandra‬ #biotechnology #Yeast #Two-Hybrid #Assay & Setup | #DNA-Binding #Domain (DBD) & #Activation Domain (AD) #Y2H #Y2HS #Bait #Prey    • Part 1 – Phage Display: Introduction & Bas...      • Part 2 – METHODS : Display of a Test Prote...      • Part 3 – VECTORS: Bacteriophage & Phagemid...   #biotechnology #csirlifesciences #msc #bsc #rpsc #uppsc #mppsc #psc #zoology #botany #lifesciences #dbt #gatexl #dna #rna In this video, we begin our series on the Yeast Two-Hybrid (Y2H) assay, a powerful technique used to study protein–protein interactions (PPI). This part focuses on the basic setup of the system and the role of the DNA-Binding Domain (DBD) and Activation Domain (AD). 🔬 Topics Covered: Introduction to Yeast Two-Hybrid assay Principle of transcription factor reconstitution Structure of a transcription factor DNA-Binding Domain (DBD) Activation Domain (AD) Concept of bait and prey proteins Reporter gene activation Basic experimental setup in yeast 📌 Key Concept: When two proteins interact, the DBD and AD are brought together, reconstituting a functional transcription factor that activates a reporter gene. 🎯 Applications: Identification of unknown interacting proteins Mapping protein interaction networks Functional genomics studies This video is helpful for Biotechnology, Life Sciences, BSc, MSc, CSIR-NET, GATE, and research students. Stay tuned for Part 2, where we will discuss reporter genes and screening strategies in detail. If you want, I can also prepare: