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Watch the Full Video at https://www.jove.com/v/59072/microele.... Microelectrode Impalement Method to Record Membrane Potential from a Cannulated Middle Cerebral Artery - a 2 minute Preview of the Experimental Protocol Joey T. Reed, Sumit P. Sontakke, Mallikarjuna R. Pabbidi University of Mississippi Medical Center, Department of Pharmacology and Toxicology; The primary goal of this article is to provide details of how to record membrane potential (Vm) from the middle cerebral artery using the microelectrode impalement method. The cannulated middle cerebral artery is equilibrated to gain myogenic tone, and the vessel wall is impaled using high resistance microelectrodes. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments