Assessing Antigen-Specific T-Cell Functionality with Dendritic Cell/CD8⁺ T Cell Co-culture скачать в хорошем качестве

Assessing Antigen-Specific T-Cell Functionality with Dendritic Cell/CD8⁺ T Cell Co-culture

In this webinar, learn how to set up DC and CD8⁺ T cell co-culture experiments and assess T cell proliferation and activity. Dendritic cells (DCs) are powerful antigen-presenting cells that process exogenous antigens and present them to CD4⁺ and CD8⁺ T cells to generate immune responses. The presentation of exogenous antigens on major histocompatibility complex (MHC) class I molecules to CD8⁺ T cells is known as cross-presentation and is essential to induce antigen-specific responses of CD8⁺ T cells to fight infections and disease. Therefore, co-culturing DCs and CD8⁺ T cells is a valuable method for studying antigen presentation and T cell activation. Using co-cultured T cells, further studies can be conducted on the presentation of tumor antigens, T cell-mediated cytotoxicity, and memory T cell development. Such studies can help develop cancer treatments and vaccines that harness T cell functions to fight tumors and induce long-lasting immune memory for protection against cancer. In this webinar, Dr. Catherine Ewen from STEMCELL Technologies will describe, in detail, how to set up DC and CD8⁺ T cell co-culture experiments that generate antigen-specific CD8⁺ T cells, and how to assess CD8⁺ T cell proliferation, functionality, and killing activity. Key Topics Include: Learn how to use DC/CD8⁺ T cell co-culture to study the antigen-specific responses of CD8⁺ T cells, including their activation, proliferation, cytokine production, and cytotoxic killing activity. Explore the features of STEMCELL’s ImmunoCult™ Medium and Activators. Isolate antigen-specific cells with EasySep™ Release.