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Watch the Full Video at jove.com/v/59714/assessing-cell-viability-... . Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells - a 2 minute Preview of the Experimental Protocol Michala G. Rolver, Line O. Elingaard-Larsen, Stine F. Pedersen University of Copenhagen, Section for Cell Biology and Physiology, Department of Biology, Faculty of Science; Here, we present several simple methods for evaluating viability and death in 3D cancer cell spheroids, which mimic the physico-chemical gradients of in vivo tumors much better than the 2D culture. The spheroid model, therefore, allows evaluation of the cancer drug efficacy with improved translation to in vivo conditions. Visit jove.com/?utm_source=youtube&utm_medium=so... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments