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Operation Theatre (OT) culture

#otculture Operation Theatre (OT) swab aerobic culture test What is Operation Theatre (OT) swab aerobic culture test? The OT swab culture test is done to check for and identify aerobic bacteria on different equipment and surfaces in an operation theatre. It is mainly done to determine if the OT is free from harmful microbes and thus clean enough for use. Aerobic bacteria are those that need oxygen to grow, so they can grow on any unclean and exposed surface. Surgical procedures require a specific level of sterility that needs to be maintained in the OT. If the OT is contaminated in any way, it significantly increases the risk of surgical site infections in a patient. Nearly 30% of people who visit any medical facility in India acquire nosocomial infections (infections acquired in the hospital), as compared to the 5% in western counties. Also, nosocomial infections are often caused due to microbial contamination of the OT. Commonly, infections that occur after surgery can be caused by bacteria such as Staphylococcus, Streptococcus or Pseudomonas. These germs can transmit through contaminated surgical instruments or through the air in OTs. Why is an Operation Theatre (OT) swab aerobic culture test performed? How do you prepare for an Operation Theatre (OT) swab aerobic culture test? How is an Operation Theatre (OT) swab aerobic culture test performed? What do Operation Theatre (OT) swab aerobic culture test results mean? Why is an Operation Theatre (OT) swab aerobic culture test performed? This test is performed as a surveillance procedure to control surgical infections (infections caused after surgery) due to the micro-organisms present in the OT. The test provides data on the type and count of the microbes in the OT and ascertains if there is a need for disinfection. Nosocomial infections are a considerable cause of morbidity and mortality in the world. Surgical site infections are the most common cause of nosocomial infections, second only to urinary tract infections. The infection can be acquired from many different sources such as the air, surface or various articles in the OT. The occurrence of a post-operative infection depends on different factors as follows: Bacterial contamination of the OT air Effective sterilisation procedures in the OT OT design Surgical technique OT discipline Prophylactic use of antibiotics Post-operative infections due to the contamination from micro-organisms in the OT can have serious and long-lasting effects on the person and their families. Infections can lead to: Extended hospital stays Increased resistance to anti-microbial medicines Long-term disability Additional financial burden on healthcare systems Death Therefore, environmental and instrument sterilisation of the OT requires monitoring. How do you prepare for an Operation Theatre (OT) swab aerobic culture test? The OT should be disinfected and sterilised before the swabs are taken. How is an Operation Theatre (OT) swab aerobic culture test performed? Sterile swabs are used for sample collection. The swabs are soaked in nutrient broth. At a bare minimum, swabs from the following sites are required: Upper surface of the OT table Lower glass surface of the OT lights Any two walls from a location above the OT table height Floor: two samples, one on either side of the OT table Surface of the trolleys used to carry sterile instruments Anaesthesia machine (swab the area where medications are placed during use) Air conditioner outlet Once the swabs are collected, the OT is closed for the day and no one is allowed to enter. The swabs are labelled and sent immediately to the laboratory for processing. On the second and third day, fresh swabs are collected from the same surfaces and sent to the lab for analysis. What do Operation Theatre (OT) swab aerobic culture test results mean? Normal results: No growth of any micro-organisms or sparse growth of harmless micro-organisms (that are found on the human skin) in any of the cultures is considered normal. It indicates that the OT can now be used. Abnormal results: If there is any growth of aerobic bacteria in the culture, it means that the OT cannot be used and needs to be cleaned and disinfected again. Once the cleaning and disinfection procedure is complete, the test should be repeated. Some commonly found bacteria that can potentially cause infections are: #Bacillus spp. #Staphylococcus aureus #Coagulase-negative #Staphylococcus #Enterococcus spp. ot ko fumigate kese kare How do you do an OT culture? How is an Operation Theatre (OT) swab aerobic culture test performed? Upper surface of the OT table. Lower glass surface of the OT lights. Any two walls from a location above the OT table height. Floor: two samples, one on either side of the OT table. Surface of the trolleys used to carry sterile instruments. What is OT swab test? How do you collect OT swab samples for microbiological testing?