Somaclonal Variation, Mutation, and Epigenetics скачать в хорошем качестве

Somaclonal Variation, Mutation, and Epigenetics

How is it possible that some clones change behavior over time? Chris Leavitt, founder, Voyager Genetics Anna Schwabe, PhD Plant Genetics Austin Sheppe, PhD Plant Genetics Oussama Badad, PhD Plant Genetics AMP Jones, PhD Biology We referenced a lot of concepts and papers during the talk- here is a significant set of reading you can do on these various subjects if you want to learn more! Cannabis-Specific Research Reviewed and highlighted morphological, cellular, and epigenetic changes related to developmental phases and secondary metabolite biosynthesis pathways during vegetative propagation https://www.sciencedirect.com/science/arti... Results suggest that basal-derived cannabis explants should be used preferentially as starting material for in vitro propagation https://www.mdpi.com/2079-7737/12/3/443 Results provide comprehensive insights into gene expression dynamics and molecular mechanisms underlying the development of diverse cannabis calli. https://www.mdpi.com/1422-0067/24/19/14625 Mutations in clones https://atrium.lib.uoguelph.ca/items/941bd... Cloning stress matters: Cutting, rooting, nutrient variation, and temp swings can trigger epigenetic shifts. https://www.sciencedirect.com/science/arti... Tissue culture methods preserve DNA methylation patterns, reducing variability in cannabis clonal lines. Boissinot, J. et al. 2024. Quick Science Background Epigenetics = Heritable but reversible gene expression changes. https://www.pagepress.org/journals/index.p... https://pubmed.ncbi.nlm.nih.gov/25800661/ https://pubmed.ncbi.nlm.nih.gov/21515434/ In clonal plants like cannabis, there's no meiosis to reset these changes, so they can stick around and even be passed to the next clone. https://www.sciencedirect.com/science/arti... These changes can be adaptive, helping plants respond to stress and improve over time. https://onlinelibrary.wiley.com/doi/epdf/1... Epigenetic Memory in Plants Plants can "remember" stress and prep their offspring to handle it better. Bruce et al. 2007 Example: Mimulus guttatus grew more trichomes (natural defense) after fake herbivore attacks- and passed it to offspring! Scoville et al. 2011 Dandelions hit by simulated herbivory passed down stunted growth and methylation patterns for 3 generations. Verhoeven et al. 2010 Stress- Induced Flowering https://academic.oup.com/jxb/article/67/17... Stress and Epigenetics https://www.mdpi.com/2223-7747/13/2/163 https://link.springer.com/article/10.1007/... Somaclonal Variation https://link.springer.com/article/10.1007/... https://www.mdpi.com/2073-4395/13/3/730