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The ChUG group talks about what we would have seen at CYTO 2020 but saw on a bunch of webinars instead. David Leclerc - CAT Facility Technical Direct (University of Chicago) Patricia Simms - Flow Cytometry Core Facility Manager (Loyola University) Laura Johnston - CAT Facility Associate Scientific Director (University of Chicago) Ryan Duggan - Senior Scientist (AbbVie) Introductions (1:10) Covid-19 impact on the labs in Chicago (4:37) Revisiting ChUG (13:44) The Season of Webinars (19:02) AACR / Technical considerations / People in monkey suits / CYTO online The SONY ID7000 Spectral flow cytometer (34:48) What to do with new lasers / Adjusting laser power or detector gain / How to buy an instrument The Propel Labs Bigfoot (51:03) The Propel Labs history / Plate sorting made very quick / Considerations on unmixing The Miltenyi MACSQuant Tyto (1:10:03) It is not like the Calibur / High speed valves / Sample preparation is everything / the joy of bad practices in flow cytometry / Flow Cytometers paint jobs The new Phitonex dyes (1:27:33) Structures considerations / Single laser excitation / FRET made great / Brightness control in panel design / Phitonex and the new laser lines No one wants to talk about the BD vs. Cytek lawsuit, we're out (1:42:48)