Transglutaminase in Clathrin Receptor Mediated Endocytosis: Crosslinking of clathrin? скачать в хорошем качестве

Transglutaminase in Clathrin Receptor Mediated Endocytosis: Crosslinking of clathrin?

1) TRANSDAB: http://genomics.dote.hu/wiki/index.ph... Clathrin heavy chain is a transglutaminase substrate. 2) TUCCIARONE ‎1981: http://www.sciencedirect.com/science/... It appears that there is a step prior to internalization in receptor-mediated endocytosis that involves the clustering of receptor-bound ligands to the coated pit regions of the membrane. This clustering process is believed to be catalyzed by transglutaminase, an enzyme that forms E-(y-glutamyl)lysine crosslinks between protein molecules. This proposal is based upon the observation that alkylamines and certain lysine-containing peptides that inhibit transglutaminase activity also inhibit migration of receptor-bound ligands to the coated pits and thereby inhibit internalization. In addition, both the endocytotic process and the transglutaminse enzyme require the presence of extracellular Ca+2 ions. Inhibition of transglutaminase activity appears to interfere with internalization by inhibiting a step prior to uptake. This step involves the migration of receptor-bound ligands into the coated pit regions of the cell membrane. The authors have proposed a model whereby the presence of a Ca+2-activated transglutaminase would lead to a crosslinking of proteins in the ligand-receptor complexes clustered in the coated pit, the result of which would be to make this aggregation irreversible. 3) WARD 1984: https://www.ncbi.nlm.nih.gov/pubmed/6... Binding of a protein ligand to its receptor at the surface of a clathrin-coated pit is thought to be stabilized by the formation of a covalent E-(y-glutamy1)-lysine cross-bridge by the action of cell surface transglutaminase.This enzyme is inhibited by primary amines including monodansylcadaverine and amantadine. 4) KOHNO 1985: https://www.jstage.jst.go.jp/article/... ...receptor-mediated endocytosis, which consists of several steps, i.e. the clustering of the receptor-ligand complex in clathrin-coated pits, crosslinking between clathrin molecules by transglutaminase and the formation of coated vesicles. Monodansylcadaverine is a highly potent inhibitor of transglutaminase. The enzyme catalyzes the formation of crosslinks between clathrin molecules at coated pits when a ligand is bound to its receptor. 5) SCHAPIRO 1998: https://www.ncbi.nlm.nih.gov/pubmed/9... Initial studies in Daudi cells showed that monodansyl cadaverine prevents endocytosis of the B subunit of verotoxin 1 (VT1B). This alkyl amine competitively inhibits cytosolic transglutaminase activity, an enzyme involved in the cross-linking of ligand-bound cell surface receptors clustered within clathrin-coated pits. 6) SCHUTZE 1999: https://www.ncbi.nlm.nih.gov/pubmed/1... MDC (monodansylcadaverine) is an inhibitor of transglutaminase, a membrane-bound enzyme that actively participates in internalization of various receptor systems. Transglutaminase, an enzyme crucially involved in coated pit formation, the initial step in receptor endocytosis. MDC has been extensively used to block endocytosis and trafficking of various ligand-receptor systems. MDC is known as a potent competitive inhibitor of transglutaminase. The enzymatic action of transglutaminase involves cross-linking of proteins by forming an isopeptide bond between a lysine residue of one protein and a glutamine residue of another protein during coated pit formation. MDC has been shown to block endocytosis of α2-macroglobulin and many polypeptide hormones and cytokines like epidermal growth factor, hepatocyte growth factor, insulin-like growth factor-I, and IL-8. MDC-mediated inhibition of transglutaminase has also been implicated in the blocking of endocytosis of vesicular stomatitis virus, Semliki Forest virus, and other types of endocytosis occurring through clathrin-coated vesicles. A previous study by Davies and co-workers, reporting on MDC-mediated inhibition of α2-macroglobulin receptor clustering. Because the effects of MDC inhibition are presumably based on a blockade of protein cross-linking mediated by transglutaminase... 7) LOWTHER 2011: https://www.ncbi.nlm.nih.gov/pubmed/2... MDC (monodansylcadaverine) inhibits the protein cross-linker, tissue transglutaminase, which is thought to inhibit the clustering and internalization of clathrin. The effect of MDC is likely to be related to inhibition of the protein cross-linker, tissue transglutaminase, which is thought to inhibit the clustering and internalization of clathrin. Indeed, MDC does not inhibit endocytosis in cells lacking transglutaminase.