Lecture On Urea Estimation: GLDH Kinetic Method Explained | MBBS | TSMC скачать в хорошем качестве

Lecture On Urea Estimation: GLDH Kinetic Method Explained | MBBS | TSMC

This video provides a detailed explanation of the GLDH kinetic method used for urea estimation, covering the principles, procedure, calculations, clinical significance, and technical specifics. Learn about the principle of the GLDH kinetic method, which involves a two-step reaction [00:21]. In the first step, urea reacts with water in the presence of urease to form CO2 and ammonia [00:25]. In the second step, the ammonium ion reacts with α-ketoglutarate and NADH in the presence of Glutamate Dehydrogenase (GLDH) to form L-glutamate and NAD+ [00:56]. The rate of this reaction is calculated by observing the oxidation of NADH to NAD+ [02:11]. Understand the procedure for urea estimation using a semi-auto analyzer [02:52]. The video details the preparation of blank, standard, and test solutions, specifying the quantities of working reagent, distilled water, standard solution, and serum sample [03:17]. As a kinetic method, no incubation period is required [04:30]. Absorbance readings are taken at specific time points to calculate the reaction rate [05:04]. The calculations for urea estimation are explained, noting that the formula is similar to that used for creatinine [05:49]. The formula involves the change in absorbance of the test sample divided by the change in absorbance of the standard, multiplied by the standard concentration [06:15]. The concept of clearance is introduced, and the general formula (U x V / P) for calculating the clearance of various analytes, including urea, is provided [07:02]. Explore the clinical aspects of urea levels [08:05]. The video discusses the causes of increased urea levels, categorizing them into pre-renal, renal, and post-renal causes. It also briefly mentions causes of decreased urea levels, such as liver cirrhosis [10:03]. The video provides normal and expected values for urea levels, including a range of 10 to 50 mg/dL and a more specific range of 16 to 38 mg/dL [11:52]. Understand the method specifics of the GLDH kinetic method [12:46], including its linearity up to 200 mg/dL of urea, the ability to detect as low as 1 mg/dL [13:05], and the filter wavelength used (340 nanometers) [13:49]. TRIPURA SANTINIKETAN MEDICAL COLLEGE Real Class room Demonstration/Lecture On: (BIOCHEMISTRY) ESTIMATION OF UREA USINGH SEMIAUTO ANALYZER BY- DR. KARAN PAL SINGH (MBBS Batch: 2024- 2025) Conducted on:- 21-03-25