Finding the Right Biochemical Assay for HTS and Lead Discovery скачать в хорошем качестве

Finding the Right Biochemical Assay for HTS and Lead Discovery

Finding the right enzyme activity assay is crucial for drug discovery. Transcreener is ultra-sensitive, simple, robust, and accurate, making it easily scalable for high-throughput screening (HTS) and flexible enough to run in kinetic mode for streamlined assay development and SAR studies. Transcreener had advantages in: Sensitivity Simplicity Robustness Flexibility HTS Compatibility Transcreener uses patented technology that employs a highly selective antibody and tracer to measure a common enzyme product. Examples of these products include ADP for kinases and ATPases, GDP for GTPases, and cGAMP for cGAS, among others. By measuring a common product, Transcreener is a universal assay system that can be quickly adapted to different enzymes. The assays offer three fluorescent readouts: fluorescence polarization (FP), fluorescence intensity (FI), and time-resolved fluorescence resonance energy transfer (TR-FRET). Choose the one that best suits your needs and your preferred plate reader. The sensitivity of Transcreener allows you to use less enzyme, saving thousands of dollars during screening and enabling accurate detection of both weak and potent inhibitors. Simplicity is essential for HTS preparation. Assays that require only a single homogeneous addition and no wash steps save hours or even days during large-scale screens. This approach also reduces mistakes that can occur during automation and minimizes human error. You simply run your enzyme reaction, mix and read, then collect your data. Transcreener is extremely robust. Reagents have been tested on the deck and in plates, with measurements taken up to 24 hours after the reaction. Our ADP FP assay reagents have maintained stability even after 21 days at room temperature! The flexibility of the assays allows them to be used in either stop or real-time mode. Kinetic mode has advantages for assay development and applications such as residence time determination. You can easily scale Transcreener from 96 wells to 1536 wells or more; just be sure to keep the reagent ratios consistent. Since Transcreener measures a common product, it can be used with virtually any substrate, eliminating the need for non-native substrates that other continuous assay methods require. When searching for the right enzyme activity assay for HTS, Transcreener provides a simple, flexible, and robust option to identify the most accurate hits for your screen.