Endocardial and Coronary Endothelial Cells Isolation from Heart | Protocol Preview скачать в хорошем качестве

Endocardial and Coronary Endothelial Cells Isolation from Heart | Protocol Preview

Watch the Full Video at https://www.jove.com/v/61126/isolatio.... Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart - a 2 minute Preview of the Experimental Protocol Alyssa Klein, Bethel Bayrau, Yifei Miao, Mingxia Gu Stanford School of Medicine, Department of Pediatrics, Division of Cardiology; Stanford School of Medicine, Vera Moulton Wall Center for Pulmonary Vascular Disease; Stanford School of Medicine, Stanford Cardiovascular Institute; Cincinnati Children's Hospital Medical Center, Division of Pulmonary Biology, Department of Pediatrics; Cincinnati Children's Hospital Medical Center, Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute; We present a protocol for the isolation of endocardial and coronary endothelial cells from rat hearts through sequential tissue digestion in a digestion buffer, cell collection from recurrent centrifuge cycles, and cell purification using anti-rat CD31 microbeads. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments