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Watch the Full Video at https://www.jove.com/v/3263/basophil-.... Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity - a 2 minute Preview of the Experimental Protocol Markus Steiner, Andrea Harrer, Roland Lang, Michael Schneider, Fátima Ferreira, Thomas Hawranek, Martin Himly University of Salzburg, Department of Molecular Biology; Paracelsus Medical University, Department of Neurology; Paracelsus Medical University, Department of Dermatology; Bühlmann Laboratories,; University of Salzburg, Christian Doppler Laboratory for Allergy Diagnosis and Therapy; Basophil activation test is a potent tool for the detection of IgE-dependent allergies in vitro. Here, an optimized protocol for basophil activation test is used to investigate drug hypersensitivity. A method for the efficient production of covalent drug-protein conjugates and their physicochemical characterization is described. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments