Absolute Gene-ius S4E2 Science Snapshot: When PCR gets dirty - tackling PCR inhibitors скачать в хорошем качестве

Absolute Gene-ius S4E2 Science Snapshot: When PCR gets dirty - tackling PCR inhibitors

PCR inhibitors are everywhere and they can definitely mess with your data. From muddy lake water to complex viral vector preparations, inhibition is a reality that can’t be ignored. In this inaugural Absolute Gene-ius: Science Snapshot, hosts Jordan Ruggieri and Lisa Crawford revisit standout moments from past seasons to explore how scientists across disciplines deal with PCR inhibition. Through clips from experts working in wastewater surveillance, environmental microbiology, and gene therapy manufacturing, the episode highlights why inhibitors distort qPCR results and how sample preparation alone doesn’t always solve the problem. Guests including Ray Ketchum, Sarah Philo, Patrick Hanington, Dave Bauer, Kimberly Gomez, and Min Jin Kim explain both the biological sources of inhibition and the technical reasons digital PCR is more resilient. Together, these perspectives paint a clear picture of why dPCR is becoming the method of choice when overcoming inhibitors is required.