SUTURING OF AMNIOTIC MEMBRANE IN CASES OF PERSISTENT EPITHELIAL DEFECT скачать в хорошем качестве

SUTURING OF AMNIOTIC MEMBRANE IN CASES OF PERSISTENT EPITHELIAL DEFECT

The amniotic membrane is the inner layer of the fetal membrane. The amniotic membrane has been shown to have anti-inflammatory, anti-fibrotic, and anti-angiogenic properties. Its transparent structure provides an excellent substrate for the growth, migration, and adhesion of epithelial corneal and conjunctival cells. STEPS: ========= Amniotic membrane along with nitrocellulose paper is cut according to the size of the epithelial defect. Amniotic membrane is peeled off from the nitrocellulose paper and placed the stromal side down (the side which is attached to the nitrocellulose paper) and ensure that amniotic membrane is spread evenly across the cornea without folds Take a partial thickness, long bite suture with 10-0 nylon suture While entering, the needle is kept vertical (perpendicular to the surface) then it is rotated into the stroma so that it travels 4-6mm at the depth of 50%-70% of the stroma The needle tip should be vertical when it comes ou Amniotic membrane is held with a plain of forceps to prevent the crumpling The knot is tied in a 3-1-1-1 pattern Take the second suture diagonally opposite to the first suture (similarly to the first suture) Similarly, all sutures are taken Amniotic membrane nicely spread and all sutures knots are buried Can excise any excess amniotic membrane then place the BCL