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Watch the Full Video at https://www.jove.com/v/52686/three-di.... Three-Dimensional (3D) Tumor Spheroid Invasion Assay - a 2 minute Preview of the Experimental Protocol Maria Vinci, Carol Box, Suzanne A. Eccles The Institute of Cancer Research, Division of Molecular Pathology; The Institute of Cancer Research, Division of Cancer Therapeutics; Invasion of surrounding normal tissues is a defining characteristic of malignant tumors. We provide here a simple, semi-automated micro-plate assay of invasion into a natural 3D biomatrix that has been exemplified with a number of models of advanced human cancers. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments