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Watch the Full Video at https://www.jove.com/v/54042/extended.... Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment - a 2 minute Preview of the Experimental Protocol Allison S. Harney, Yarong Wang, John S. Condeelis, David Entenberg Albert Einstein College of Medicine, Department of Anatomy & Structural Biology; Albert Einstein College of Medicine, Department of Radiology; Albert Einstein College of Medicine, Gruss-Lipper Biophotonics Center; Albert Einstein College of Medicine, Integrated Imaging Program; This protocol describes the use of multiphoton microscopy to perform extended time-lapse imaging of multicellular interactions in real time, in vivo at single cell resolution. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments