Step 2: Perform Western Blot | Publishing Western Blot Data скачать в хорошем качестве

Step 2: Perform Western Blot | Publishing Western Blot Data

Step 2: Perform Western Blot Make sure you document and provide in your manuscript: Sample information Detailed descriptions of the experiment Reagents used, especially antibody information Detector used for image acquisition and acquisition settings Any process manipulations made while capturing the image -- Do not manipulate blot images Figure annotation of lanes without distorting the blot image Key observations in the figure legends The normalization strategy used and associated details Proof that you are detecting within the combined linear range of both the target and normalizing internal loading control Complete details of the statistical software and method(s) used A Note on Images During the manuscript submission process, digital images are submitted in PDF format. After the reviewer has accepted the manuscript, editors will require that authors submit high quality images in TIFF format, with 300 dpi resolution for color RGB images and 600 dpi for grayscale images. Detailed instructions can be found under the “Instructions for authors” section on each journals’ website. Western Blot Worksheet LICORbio has created a worksheet for recording all the necessary details during your Western blot experiments. Download it free at https://licor.app.boxenterprise.net/s.... About LICORbio LICORbio offers imaging systems, analysis software, and IRDye® infrared dye reagents for quantitative protein and small animal imaging. LICORbio provides an integrated solution for quantitative Western blots with its Odyssey® Infrared Imaging Systems, Image Studio™ quantification software, Empiria Studio® analysis software for publication-ready data, reagents, unique IRDye® secondary antibodies, and online training courses. For chemiluminescent Western blots, the C-DiGit® Blot Scanner is a personal, portable imager. Use the Odyssey® XF or Odyssey M Imaging Systems for both chemiluminescence and near-infrared fluorescence Western blots. You can achieve superior small animal imaging results using BrightSite™ near-infrared fluorescent probes or bioluminescence on the Pearl® Trilogy imaging system. LICORbio Website https://www.licorbio.com/ Reagent Store https://www.licor.com/bio/reagents Image Studio™ Software https://www.licor.com/image-studio LinkedIn   / licorbio   Twitter   / licorbio   Facebook   / licorbio