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Watch the Full Video at https://www.jove.com/v/2761/mouse-mod.... Mouse Model of Middle Cerebral Artery Occlusion - a 2 minute Preview of the Experimental Protocol Terrance Chiang, Robert O. Messing, Wen-Hai Chou Ernest Gallo Clinic and Research Center, University of California, San Francisco, Department of Neurology; Kent State University, Department of Biological Sciences; We demonstrate in the video a method for producing a middle cerebral artery occlusion in adult mice using an intraluminal monofilament. We also show how to evaluate the extent of cerebral infarction by 2,3,5-triphenyltetrazolium chloride (TTC) staining. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments