Gram Staining Procedure & Principle | Gram staining step by step Method | Gram & Acid-Fast Staining скачать в хорошем качестве

Gram Staining Procedure & Principle | Gram staining step by step Method | Gram & Acid-Fast Staining

Gram staining for differentiating bacterial species. Gram staining method is used for identifying unknown bacteria. The method for mounting bacteria on a microscope slide using aseptic technique is shown. Following Reagent Used in the test Crystal violet (primary stain) Iodine solution/Gram's Iodine (mordant that fixes crystal violet to cell wall) Decolorizer (e.g. ethanol) Safranin as secondary stain Water Procedure Make a slide of cell sample to be stained. Heat fix the sample. Add the primary stain (crystal violet) to the sample/slide and incubate for 1 minute. Rinse slide with a gentle stream of water for a maximum of 5 seconds to remove unbound crystal violet. Add Gram's iodine for 1 minute- Rinse sample/slide with acetone or alcohol for ~3 seconds and rinse with a gentle stream of water. Add the secondary stain, safranin, to the slide and incubate for 1 minute.