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Watch the Full Video at https://www.jove.com/v/61931/quantify.... Quantifying Fibrillar Collagen Organization with Curvelet Transform-Based Tools - a 2 minute Preview of the Experimental Protocol Yuming Liu, Kevin W. Eliceiri University of Wisconsin-Madison, Laboratory for Optical and Computational Instrumentation, Center for Quantitative Cell Imaging; University of Wisconsin-Madison, Departments of Medical Physics and Biomedical Engineering; Morgridge Institute for Research, Madison, Wisconsin,; test, test; Here, we present a protocol to use a curvelet transform-based, open-source MATLAB software tool for quantifying fibrillar collagen organization in the extracellular matrix of both normal and diseased tissues. This tool can be applied to images with collagen fibers or other types of line-like structures. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments