Cap-binding Proteins Anlysis in Cells Oxygen Conditions Exposed Cells | Protocol Preview скачать в хорошем качестве

Cap-binding Proteins Anlysis in Cells Oxygen Conditions Exposed Cells | Protocol Preview

Watch the Full Video at https://www.jove.com/v/55112/analysis.... Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions - a 2 minute Preview of the Experimental Protocol Sara Timpano, Gaelan Melanson, Sonia L. Evagelou, Brianna D. Guild, Erin J. Specker, James Uniacke University of Guelph, Department of Molecular and Cellular Biology; Here, we present human cell culture protocols to analyze translation initiation factors that bind the 5' cap of mRNA during physiological oxygen conditions. This method utilizes an Agarose-linked m7GTP cap analog and is suitable to investigate cap-binding factors and their interacting partners. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments