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Tissue culture means cloning and micro-propagation of tissues of the selected Elite plants and daughter suckers. The process consists of five important steps: Initiation, Multiplication, Shooting & rooting, Primary Hardening in green houses and Secondary Hardening in shade houses. Strict adherence to aseptic standards and micro-climatic conditions and care during the hardening process alone can ensure success. The Jain Tissue Culture laboratory has successfully micro-propagated and sold over 2 million ‘Grand Nain’ banana plants with outstanding success. It is the largest Banana Tissue Culture laboratory in the country. The farmers have increased their productivity from an average of 12 kg to over 26 kg per bunch per year and have consecutively taken crops with two ratoons in about 30 months.