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Watch the Full Video at https://www.jove.com/v/57198/generati.... Generation of Size-controlled Poly (ethylene Glycol) Diacrylate Droplets via Semi-3-Dimensional Flow Focusing Microfluidic Devices - a 2 minute Preview of the Experimental Protocol Yan Wu, Xiang Qian, Shengli Mi, Min Zhang, Shuqing Sun, Xiaohao Wang Tsinghua University, Graduate School at Shenzhen; Tsinghua University, The State Key Laboratory of Precision Measurement Technology and Instruments; Here, we present a protocol to illustrate the fabrication processes and verifying experiments of a semi-three-dimensional (semi-3D) flow-focusing microfluidic chip for droplet formation. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments