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Exocytosis using Fluorescence

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching- Experimental Protocol Watch the full video at https://www.jove.com/cn/v/3747/latera..., no sign up needed for open access content, or request a trial to get instant access to our full library. This report describes the use of live cell imaging and photobleach techniques to determine the surface expression, transport pathways and trafficking kinetics of exogenously expressed, pH-sensitive GFP-tagged proteins at the plasma membrane of neurons. Visit https://www.jove.com/?utm_source=yout... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard and the University of Cambridge, benefit from using JoVE’s extensive library of 14000 videos in their research and teaching. Follow JoVE on Twitter:   / jovejournal   Like JoVE on Facebook:   / jovejournal   Subscribe to our channel:    / jovejournalofvisualizedexperiments