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Watch the Full Video at https://www.jove.com/v/55628/in-ovo-e.... In Ovo Electroporation in the Chicken Auditory Brainstem - a 2 minute Preview of the Experimental Protocol Ting Lu, Ariel Loren Cohen, Jason Tait Sanchez Northwestern University, Roxelyn and Richard Pepper Department of Communication Sciences and Disorders; Northwestern University, Knowles Hearing Research Center; Northwestern University, Department of Neurobiology; Auditory brainstem neurons of avians and mammals are specialized for fast neural encoding, a fundamental process for normal hearing functions. These neurons arise from distinct precursors of embryonic hindbrain. We present techniques utilizing electroporation to express genes in the hindbrain of chicken embryos to study gene function during auditory development. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments