Electroporation in mouse embryos enables precise gene delivery at specific developmental stages скачать в хорошем качестве

Electroporation in mouse embryos enables precise gene delivery at specific developmental stages

Reference: https://app.jove.com/t/3024/mouse-ute... Mouse in utero electroporation is a sophisticated technique utilized for precise spatiotemporal gene transfection, allowing researchers to manipulate gene expression during embryonic development. This method involves the application of electrical pulses to the developing mouse embryo, which facilitates the introduction of exogenous DNA into targeted cells. By controlling the timing and location of the electroporation, scientists can achieve specific gene modifications that are crucial for studying gene function, developmental processes, and disease mechanisms. The ability to transfect genes at various stages of embryogenesis provides invaluable insights into the roles of specific genes in development and can lead to advancements in genetic therapies. The procedure typically begins with the identification of the desired embryonic stage for intervention, often targeting the early stages of development when cells are still pluripotent. Following this, a plasmid containing the gene of interest is injected into the embryonic tissue, usually within the neural tube or other critical regions. The electroporation device then delivers a series of controlled electrical pulses, which create temporary pores in the cell membranes, allowing the plasmid DNA to enter the cells. This technique not only enhances the efficiency of gene transfer but also minimizes potential damage to surrounding tissues, making it a preferred method for in vivo studies. Moreover, the versatility of in utero electroporation extends beyond simple gene delivery; it can also be employed to study gene interactions, signaling pathways, and the effects of gene knockdown or overexpression in a living organism. Researchers can utilize this method to create models of human diseases, investigate developmental disorders, and explore the potential for regenerative medicine applications. As the field of genetic engineering continues to evolve, in utero electroporation stands out as a powerful tool that bridges the gap between basic research and therapeutic development, offering a deeper understanding of genetic functions in a dynamic biological context.