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Watch the Full Video at https://www.jove.com/v/3894/saliva-sa.... Saliva, Salivary Gland, and Hemolymph Collection from Ixodes scapularis Ticks - a 2 minute Preview of the Experimental Protocol Toni G. Patton, Gabrielle Dietrich, Kevin Brandt, Marc C. Dolan, Joseph Piesman, Robert D. Gilmore Jr. Centers for Disease Control and Prevention, Microbiology and Pathogenesis Activity, Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases; Centers for Disease Control and Prevention, Tick-Borne Diseases Activity, Bacterial Diseases Branch, Division of Vector-Borne Diseases, National Center for Emerging and Zoonotic Infectious Diseases; The collection of infected tick hemolymph, salivary glands, and saliva is important to study how tick-borne pathogens cause disease. In this protocol we demonstrate how to collect hemolymph and salivary glands from feeding Ixodes scapularis nymphs. We also demonstrate saliva collection from female I. scapularis adults. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments