Leica SP8 Confocal Tutorial - Cell Imaging Facility скачать в хорошем качестве

Leica SP8 Confocal Tutorial - Cell Imaging Facility

Watch this tutorial carefully in preparation for your in-person confocal training at the Leica SP8. You will be expected to perform all sections of this video autonomously during your training. TIMESTAMPS: 0:00 Intro 0:13 Start-up 0:45 Start software 1:42 Software overview 3:57 Load sample with a 10X (dry) objective 4:43 Set-up light path 5:32 Adjust channels 7:18 Compare different imaging conditions with the 10X objective 8:44 Z-stacks 10:47 Navigator 13:58 Switch to 25X (water immersion) objective and acquire images 17:34 3D reconstruction 18:03 Lightning 18:53 Apply image settings from previous image 19:08 Acquire z-stack with Lightning 20:29 Finishing up session and saving data 21:15 Clean up water immersion objectives 21:42 Loading sample to and cleaning up oil immersion objective lenses We thank PhD student Angie Chen from the Kristi Baker lab (University of Alberta) for providing the imaged sample! Starring Dr. Xuejun Sun. Video content and editing by Dr. Tatjana Golovin.