Brain Specific Knockdown of miRNA by Stereotactic Injection | Protocol Preview скачать в хорошем качестве

Brain Specific Knockdown of miRNA by Stereotactic Injection | Protocol Preview

Watch the Full Video at https://www.jove.com/v/53307/a-simple.... A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA - a 2 minute Preview of the Experimental Protocol Hemant Suryawanshi, Mayuresh Anant Sarangdhar, Manika Vij, Reema Roshan, Vijay Pal Singh, Munia Ganguli, Beena Pillai CSIR-Institute of Genomics and Integrative Biology, New Delhi, India,; Academy of Scientific and Innovative Research (AcSIR), New Delhi, India,; MicroRNAs play crucial roles in the brain and are potential targets for modeling neuro-degeneration. However, perturbing miRNA levels is challenging due to the short length of miRNA and inaccessibility of the brain tissue. This video presents a method for antagomir design and brain specific delivery using a neuropeptide in mice. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments