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Accelerating strain development process for CDMO and CRO companies

Presented By: Eric Abbate Speaker Biography: Throughout my academic career, I have always been passionate about how quick bacteria can adapt to difficult environments. In the context of antibiotic treatments, I focussed on “persisters”, a kind of developmental state of cells with high level of antibiotic tolerance. As one of the first to approach persistence from an evolutionary point of view, I identified the fast tuning of this ‘bet-hedging’ behaviour (populations are said to hedge their bets against potential future disasters of antibiotic encounters by producing more/less persisters). Populations under frequent treatment quickly accumulate small and unexpected mutations that reroute physiology to overcome antibiotic-induced killing in previously unknown target genes. Later we have shown that such a fast evolutionary response of antibiotic tolerance is also true in all of the ESKAPE pathogens and that it precedes and catalyses the development of genetic resistance. Currently, we are further examining how the evolution of persistence and resistance intertwines, making use of high-throughput assays, and how the evolved mechanisms are adjusted to tune the level of surviving persister cells. Webinar: Accelerating strain development process for CDMO and CRO companies Webinar Abstract: Manufacturing of proteins, mRNA or small molecules requires comprehensive strain engineering approaches to increase yield, productivity, and robustness of the strain. While some of these goals can be accomplished with standard genetic engineering methods, others require a whole-genome, exploratory approach that can take many months. With the Onyx® Digital Genome Engineering platform, you can go after each of these objectives simultaneously by generating diverse strain libraries in a simple, automated workflow. Insertions, deletions, and substitutions across multiple targets, such as promoters, coding regions or global regulators, can be rapidly introduced to improve both specific functions and the overall manufacturability of the strain. In this webinar we will demonstrate how this approach helped improve production of heterologous proteins in E. coli and S. cerevisiae. LabRoots on Social: Facebook:   / labrootsinc   Twitter:   / labroots   LinkedIn:   / labroots   Instagram:   / labrootsinc   Pinterest:   / labroots   SnapChat: labroots_inc