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Watch the Full Video at https://www.jove.com/v/55211/ecotoxic.... Ecotoxicological Method with Marine Bacteria Vibrio anguillarum to Evaluate the Acute Toxicity of Environmental Contaminants - a 2 minute Preview of the Experimental Protocol Alice Rotini, Loredana Manfra, Filippo Spanu, Marco Pisapia, Anna Maria Cicero, Luciana Migliore Institute for Environmental Protection and Research (ISPRA), Rome (Italy),; Tor Vergata University, Rome (Italy), Department of Biology; Stazione Zoologica, Anton Dohrn, Naples (Italy), Department of Biology and Evolution of Marine Organisms; This work describes a new protocol to assess the ecotoxicity of pollutants, including emerging contaminants such as nanomaterials, using the marine bacterium Vibrio anguillarum. This method allows for the determination of the LC50, or mortality, the concentration that causes a 50% decrease in bacteria culturability, after a 6 h exposure. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments