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Several webinars (one global and five regional) were hosted to mark the launch of the ABX Guide, an open-access antibiotic treatment guideline focused on pathogen-directed treatment in resource-constrained settings. The ABX Guide is accessible through an open access website. https://www.isac-abxguide.world/ These webinars delved into the relevance of the ABX Guide and the development process behind its treatment recommendations. We explored its application across various geographic regions, featuring speakers who highlighted related projects and initiatives. In this event, we have collated all the regional presentations. 00:00 Start 00:27 Overview of ISAC / The why of the Abx Guide- Prof. Heiman Wertheim 13:18 The Abx Guide: what is in it and what is not - Dr Hind Kadri AFRICA 21:54 Making the case for patient safety in stewardship practices - Prof. Natalie Schellack EUROPE 48:03 Pathogen-directed antibacterial treatment: more demanding, but so much better than empirical treatment - Prof. Pierre Tattevin THE AMERICAS 1:08:03 Point prevalence survey of antibiotic use in Latin American hospitals: are we rationally prescribing? - Prof. Gabriel Levy Hara SOUTH, SOUTH EAST ASIA 1:24:05 Stepped-wedge trial of a Lao language antibiotic prescribing app against paper guidelines - Dr Vilada Chansamouth MENA 1:51:59 AMR in the Eastern Mediterranean Region: progress, challenges and priorities - Dr Shaffi Koya