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Watch the Full Video at https://www.jove.com/v/55185/qtl-mapp.... QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii - a 2 minute Preview of the Experimental Protocol Bang Shen, Robin H. Powell, Michael S. Behnke Huazhong Agricultural University, State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine; Louisiana State University, Pathobiological Sciences, School of Veterinary Medicine; Details are presented on how QTL mapping with a whole genome sequence based genetic map can be used to identify a drug resistance gene in Toxoplasma gondii and how this can be verified with the CRISPR/Cas9 system that efficiently edits a genomic target, in this case the drug resistance gene. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments