Resuspending, diluting, & working with DNA & RNA oligos (primers you order, etc.) скачать в хорошем качестве

Resuspending, diluting, & working with DNA & RNA oligos (primers you order, etc.)

Some tips for resuspending, diluting, & working with DNA & RNA oligos Resuspend in: TE (10 mM Tris, pH 7.5 to 8.0, 1 mM EDTA); Tris (10 mM Tris-HCl, pH 8.0); or molecular biology grade water add 1μL per nmol (look on tube) for 1mM or 10μL per nmol for 100μM make dilutions of the stock to your working concentration & aliquot if you’re going to be using lots of times avoids contaminating stock, minimizes freeze-thaws, lets you keep at higher concentration which is more stable don’t do huge dilutions - instead dilute step-wise blog: https://bit.ly/oligo_working more in video & figures and these great web pages I found IDT Tips for resuspending and diluting your oligonucleotides https://www.idtdna.com/pages/educatio... Nolan Speicher, former IDT associate. Published Mar 31, 2017 Revised/updated Sep 22, 2017 My oligos have arrived: Now what? Resuspension, dilution, storage, and other tips https://www.idtdna.com/pages/educatio... Elisabeth Wagner, PhD, Manager of Scientific Applications Support, IDT. Published Jan 14, 2014Revised/updated Sep 10, 2016 Storing oligos: 7 things you should know https://www.idtdna.com/pages/educatio... Nan Pazdernik, PhD, Science Writer, IDT Nolan Speicher, former IDT associate. Published Jun 20, 2017Revised/updated Dec 3, 2020 Millipore Sigma: Oligonucleotide Handling & Stability: https://www.sigmaaldrich.com/US/en/te... more on concentrations and C1V1=C2V2: blog form: http://bit.ly/c1v1equalsc2v2 ; YouTube:    • C1V1 = C2V2 (aka M1V1=M2V2) is so useful f...   more on dimensional analysis: http://bit.ly/dimensionalanalysising &    • Dimensional analysis (aka factor-label met...   more on how these things are synthesized: http://bit.ly/solidstateoligo                       more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 http://bit.ly/2OllAB0 or search blog: http://thebumblingbiochemist.com