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The video was recorded live during the SIB course “Single cell Transcriptomics” streamed on 06-08 March 2023. The course materials can be found in the dedicated GitHub page: https://sib-swiss.github.io/single-ce... This video contains information on methods used for differential gene expression analysis among cells captured within a single-cell RNA seq experiment. Methods for marker gene identification and for differential gene expression analysis among cells from different conditions are discussed. A brief overview of enrichment analysis using over-representation analysis or GSEA is presented. Finally, some databases for pathways and gene sets are presented. -Target audience: PhD students, post-docs and researchers in life sciences who are doing bioinformatics analyses and want to get introduced to single cell transcriptomics data analysis. -Speaker: Dr. Tania Wyss, SIB Swiss Institute of Bioinformatics Any questions about this talk? Contact: Tania Wyss, Tania.Wyss@sib.swiss This video is available under the creative commons license CC-BY-4.0