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Watch the Full Video at https://www.jove.com/v/51880/ordering.... Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening - a 2 minute Preview of the Experimental Protocol Viktoria Wollrab, David Caballero, Raghavan Thiagarajan, Daniel Riveline Institut de Science et d'Ingénierie Supramoléculaires (ISIS), CNRS and Université de Strasbourg, Laboratory of Cell Physics; Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS and Université de Strasbourg, Development and Stem Cells Program; We report a device and a new method to study cells and embryos. Single cells are precisely ordered in microcavity arrays. Their 3D confinement is a step towards 3D environments encountered in physiological conditions and allows organelle orientation. By controlling cell shape, this setup minimizes variability reported in standard assays. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments