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Klebsiella, Staphylococcus, E Coli, Candida, Acinetobacter, Bacillus in Gram stain Under the Microscope Klebsiella pneumoniae in Gram-stained smear of Culture Gram-stained smear of Staphylococcus aureus from culture Escherichia coli in Gram-stained smear of Culture Acinetobacter species in Gram-stained smear of Culture Yeast cells of Candida albicans in Gram-stained smear of Culture Bacillus species in Gram-stained smear of Culture Various organisms in Gram-stained smear of culture under the microscope at various magnifications When observing samples under the microscope after a Gram stain procedure, different bacteria and yeast cells will appear differently based on their cell wall structures. Here's a summary of how each of the mentioned organisms typically appears: Klebsiella: Gram Stain Result: Gram-negative Appearance: These bacteria are typically rod-shaped, also known as bacilli. They often appear in pairs or short chains. Color: Pink/red (due to the counterstain) Staphylococcus: Gram Stain Result: Gram-positive Appearance: These are round cells, or cocci, that often appear in clusters (like grapes). Color: Purple/blue (from the crystal violet stain) E. coli (Escherichia coli): Gram Stain Result: Gram-negative Appearance: These are rod-shaped bacteria, or bacilli. They often appear singly or in pairs. Color: Pink/red Candida: Note: Candida is a genus of yeasts, so it doesn't strictly follow the Gram stain protocol in the same way bacteria do. However, if Gram-stained, the cells can sometimes retain the crystal violet stain. Appearance: Yeast cells are larger than bacteria and can vary in size and shape. Some species form pseudohyphae or true hyphae. Color: If stained, they might appear purple/blue, but a Gram stain is not the best method to identify yeasts. Acinetobacter: Gram Stain Result: Gram-negative Appearance: These bacteria are typically coccobacilli, meaning they're somewhat in-between rods and circles in shape. Color: Pink/red Bacillus: Gram Stain Result: Gram-positive Appearance: These are rod-shaped bacteria, and the name "bacillus" is often used as a general term for rod-shaped bacteria. However, when referring to the genus Bacillus, it typically means spore-forming rods. Color: Purple/blue Always remember that while the Gram stain is a valuable tool in microbiology, it's only one of many tests and methods used to identify microorganisms. Observations should always be confirmed with other tests and methods, especially in clinical settings. Klebsiella: Gram-negative Rod-shaped Bacilli Pink/red Pairs or short chains Staphylococcus: Gram-positive Cocci Clusters Purple/blue Grape-like E. coli (Escherichia coli): Gram-negative Rod-shaped Bacilli Pink/red Singly or pairs Candida: Yeast Fungal cells Pseudohyphae (in some species) Hyphae (in some species) Purple/blue (though Gram stain is not optimal for yeasts) Acinetobacter: Gram-negative Coccobacilli Pink/red Non-motile Bacillus: Gram-positive Rod-shaped Bacilli Purple/blue Spore-forming (for some species) These keywords summarize the most fundamental features of these organisms as viewed under the microscope following a Gram stain.