3D Spheroid Invasion Assay with Tumor based Matrix | Protocol Preview скачать в хорошем качестве

3D Spheroid Invasion Assay with Tumor based Matrix | Protocol Preview

Watch the Full Video at https://www.jove.com/v/59567/fully-hu.... Fully Human Tumor-based Matrix in Three-dimensional Spheroid Invasion Assay - a 2 minute Preview of the Experimental Protocol Erika Naakka, Katja Tuomainen, Henrik Wistrand, Miila Palkama, Ilida Suleymanova, Ahmed Al-Samadi, Tuula Salo University of Helsinki, Department of Oral and Maxillofacial Diseases, Clinicum, Faculty of Medicine; University of Oulu, Cancer and Translational Medicine Research Unit; Oulu University Horspital, Medical Research Center; Helsinki University Hospital,; Tumor microenvironment is an essential part of cancer growth and invasion. To mimic carcinoma progression, a biologically relevant human matrix is needed. This protocol introduces an improvement for the in vitro three-dimensional spheroid invasion assay by applying a human leiomyoma-based matrix. The protocol also introduces a computer-based cell invasion analysis. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments