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*Semen freezing* (or *sperm freezing**) is the process of preserving sperm for future use in **in vitro fertilization (IVF)* or other assisted reproductive technologies (ART). It is often done in cases where sperm might not be available at the time of the procedure (e.g., cancer treatment, military service, or medical reasons) or to store high-quality sperm for later use. Steps Involved in Sperm Freezing for IVF: 1. **Semen Collection**: Sperm is collected through masturbation (or other methods in cases of medical conditions). 2. **Analysis**: The sperm sample is analyzed for motility, concentration, and morphology to assess quality. 3. **Cryoprotectant Addition**: A cryoprotectant (chemical agent) is added to protect the sperm from damage during the freezing and thawing process. Common cryoprotectants include glycerol or dimethyl sulfoxide (DMSO). 4. **Freezing**: The sperm is gradually frozen in specialized containers (straws or vials) and stored in liquid nitrogen at extremely low temperatures (around -196°C). This process can be done in stages to avoid ice crystal formation, which could damage the sperm. 5. **Storage**: Frozen sperm can be stored for extended periods (often years) without significant degradation, allowing future use in IVF or ICSI. Advantages of Sperm Freezing: **Preservation**: It ensures that sperm can be used later, even if the man’s sperm quality declines or he becomes unavailable due to health issues or other factors. **Convenience for IVF**: It allows sperm to be available for IVF or ICSI procedures without the need for immediate collection. **Cancer Treatment**: Men undergoing treatments like chemotherapy or radiation can preserve sperm before starting treatment, as these therapies can harm sperm production. Thawing and Use in IVF: When sperm is needed for IVF or ICSI, the frozen sperm is thawed and prepared for fertilization. The thawed sperm are often tested again for motility and viability before being used for fertilization. In short, *semen freezing* is a valuable tool in fertility preservation, offering flexibility and the opportunity to store sperm for future IVF or ICSI procedures.