Genome Editing with CRISPR-Cas9 скачать в хорошем качестве

Genome Editing with CRISPR-Cas9

References/Resources:   / the_crux   All videos on Genetic Engineering:    • Genetic Engineering (Genes to Genomes)   What is CRISPR-Cas9 technology? How does CRISPR-Cas9 genome editing work? What is the mechanism of genome engineering with CRISPR-Cas9? What is a PAM sequence? Where is the PAM sequence location and what is its function? What is the protospacer adjacent motif? How does Cas9 recognize target DNA through guide RNA (gRNA) ? How does the Cas9 nuclease cut target DNA? How are the repair mechanisms (HDR, homology-directed repair and NHEJ, Non-homologous end joining) involved in repairing Cas9 double stranded DNA breaks? This video explains the mechanism of CRISPR-Cas9 in genome editing. Specifically, this video explains how cas9 finds the target DNA match through PAM sequence verification and how the guide RNA pairs with the target DNA. Towards the end, we discuss how multiple repair natural occurring double-stranded DNA break repair pathways like NHEJ (non-homologous end joining) and homology directed repair can be used in CRISPR-Cas9 genome editing, especially when making large deletions or making targeted insertions. 00:00 Cas9 Endonuclease 00:34 single-guide RNA (sgRNA) 01:13 Protospacer adjacent motif (PAM) 02:18 CRISPR-Cas9 Genome editing 04:48 Exercise 05:16 Non-homologous end joining (NHEJ) 06:30 Homology directed repair (HDR) 07:14 Cell cycle and DSB repair 07:54 Cas9 to disrupt the genome 08:12 Cas9 to delete DNA from the genome 08:34 Cas9 directed donor DNA insertion 09:17 CRISPR-Cas9 challenges CRISPR-Cas9 in Bacterial Adaptive Immunity:    • CRISPR-Cas9-mediated Adaptive Immunity Mec...   References/Resources:   / the_crux   Instagram:   / thecrux.biology   Must watch playlists All videos on Genetic Engineering:    • Genetic Engineering (Genes to Genomes)   All videos on DNA Replication:    • All about DNA Replication!   All videos on Transcription:    • All about Transcription!   All videos on Translation:    • All about Translation!   Consider supporting the growth of this channel by becoming a Patron:   / the_crux   I appreciate your support :)