Engineering & Evolution Of AAV Gene Therapy Vectors Via DNA Family Shuffling l Protocol Preview скачать в хорошем качестве

Engineering & Evolution Of AAV Gene Therapy Vectors Via DNA Family Shuffling l Protocol Preview

Watch the Full Video at https://www.jove.com/v/3819/engineeri.... Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling - a 2 minute Preview of the Experimental Protocol Eike Kienle, Elena Senís, Kathleen Börner, Dominik Niopek, Ellen Wiedtke, Stefanie Grosse, Dirk Grimm Heidelberg University, Cluster of Excellence CellNetworks, Department of Infectious Diseases, Virology; Heidelberg University, Department of Infectious Diseases, Virology; We demonstrate the basic technique to molecularly engineer and evolve synthetic Adeno-associated viral (AAV) gene therapy vectors via DNA family shuffling. Moreover, we provide general guidelines and representative examples for selection and analysis of individual chimeric capsids with enhanced properties on target cells in culture or in mice. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments