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Solutions for THC Quantitation: Separation of 4 Tetrahydrocannabinol Isomers: Δ6a/10a, Δ8, Δ9... скачать в хорошем качестве

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Solutions for THC Quantitation: Separation of 4 Tetrahydrocannabinol Isomers: Δ6a/10a, Δ8, Δ9...

Presented By: Jennifer Davis Speaker Biography: Jennifer Davis serves as a LCMS Product Coordinator for Shimadzu Scientific Instruments. Since joining Shimadzu in September 2018, she has been involved in method development using LC-MS, LC-MS/MS, and LC-QToF for a variety of market segments, including the cannabis industry. Recently she did extensive research into the use of APCI-LC-MS/MS for the analysis of pesticides commonly run by GCMS. As well as developing a LCMS based method for the quantitation of THC including chromatographic separation of four ΔTHC isomers. Prior to coming to Shimadzu Jennifer worked as a medicinal chemist utilizing a variety of synthetic and analytical techniques for novel drug discovery. Webinar: Solutions for THC Quantitation: Separation of 4 Tetrahydrocannabinol Isomers: Δ6a/10a, Δ8, Δ9, and Δ10 using LC-MS Webinar Abstract: The cannabis/hemp market continues to grow each year with more states legalizing recreational marijuana, as well as the 2018 Farm Bill removing hemp from the controlled substance list. This bill defines that any cannabis sativa L. strain with a total tetrahydrocannabinol (THC) concentration of 0.3% or less can be considered hemp and not cannabis. Due to this definition, there is an even higher demand to differentiate hemp from cannabis by determining the correct concentration of THC. More recently, there are more instances of adulterated hemp samples with THC variants and a need to fully identify and quantify each of these variants. Currently most laboratories are only monitoring and reporting delta-8 and delta-9 THC. This study develops an LCMS method that separates, identifies, and quantitates delta-8 and delta-9, as well as, delta-10 and delta-6a/10a THC. A Shimadzu LCMS mass spectrometer coupled with a modular Nexera 40 series UHPLC system and a PDA detector was employed for simultaneous analysis of analyte absorbance and compound ionization. Separation of delta-6a/10a THC, delta-8 THC, delta-9 THC, and delta-10 THC was achieved in a single chromatographic run on both a LCMS-2020 single quadrupole mass spectrometer and a LCMS-8045 triple quadrupole mass spectrometer. The LCMS-2020 utilized SIM events to monitor chromatographic separation as well as a scan event to monitor in-source fragmentation for additional analyte confirmation. The LCMS-8045 was used for a more comprehensive cannabinoid analysis allowing for detection of all 4 THC isomers plus 17 other cannabinoids. Both detection methods demonstrated the capability to simultaneously detect, confirm, and quantitate the four different ΔTHC isomers. Learning Objectives: 1. Identify the benefit to separating out the different ΔTHC isomers currently being detected in hemp and cannabis samples. 2. List alternate detection methods for cannabinoids other than UV/Vis and GC-FID. 3. Discuss method development and results when using LCMS to chromatographically separate and detect ΔTHC isomers. Earn PACE Credits: 1. Make sure you’re a registered member of LabRoots https://www.labroots.com/webinar/solu... 2. Watch the webinar on YouTube or on the LabRoots Website https://www.labroots.com/ms/webinar/s... 3. Click Here to get your PACE credits (Expiration date – March 23, 2024): https://www.labroots.com/credit/pace-... LabRoots on Social: Facebook:   / labrootsinc   Twitter:   / labroots   LinkedIn:   / labroots   Instagram:   / labrootsinc   Pinterest:   / labroots   SnapChat: labroots_inc

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